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Cellscript Inc amplicap max t7 high yield message maker kit
Amplicap Max T7 High Yield Message Maker Kit, supplied by Cellscript Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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amplicap max t7 high yield message maker kit - by Bioz Stars, 2026-10
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Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.

In Vitro:

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.

Produced:

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.

Construct:

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.

Enzyme-linked Immunosorbent Assay:

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.

Labeling:

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.

Transfection:

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.

Digital PCR:

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.

Sequencing:

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.

shRNA:

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.

Plasmid Preparation:

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.

Cloning:

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.

Real-time Polymerase Chain Reaction:

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.

Recombinant:

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.

Software:

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.

Clone Assay:

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.

Expressing:

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HBs PreS-1 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S1HP-01 HBs PreS-2 antigen quantitative ELISA kit Rapid Beacle Inc Cat#BCL-S2HP-01 mMESSAGE mMACHINETM T7 transcription kit Thermo Fisher Scientific Cat#AM1344 AmpliCap-Max T7 High Yield Message Maker Kit CellScript CatC-ACM04037 A-Plus Poly(A) Polymerase Tailing Kit CellScript Cat#C-PAP5104H Maxwell® RSC genomic DNA kit Promega Cat#AS1880 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2052 DIG RNA labeling kit (SP6/T7) Roche Cat#11175025910 GenJetTM In Vitro DNA Transfection Reagent for HepG2 Cells SignaGen Laboratories Cat#SL100489-HEPG2 Absolute QTM DNA Digital PCR Master Mix Thermo Fisher Scientific Cat#A52490 Experimental models: Cell lines Human: NTCP-expressing HepG2 cells Iwamoto et al.31 N/A Human: HepAD38.7 cells Ladner et al.27 N/A Human: LentiX HEK293T cells Clontech Cat# 632180 Oligonucleotides siRNA targeting sequence (see Table S1) This paper N/A shRNA targeting sequence (see Table S1) This paper N/A Primers for plasmid cloning (see Table S1) This paper N/A Primers for qPCR and dPCR (see Table S1) This paper N/A Recombinant DNA pcDNA3.1 Thermo Fisher Scientific Cat#V79020 pT7-pgRNA This paper N/A pT7-pgRNA poldead This paper N/A pT7-2.4kb RNA This paper N/A pT7-2.1kb RNA This paper N/A pT7-Small S RNA This paper N/A pT7-XRNA This paper N/A pRL-TK Promega Cat#E2241 pcDNA-GFP Nakayama et al.48 N/A pHBV-WT Fauzyah et al.46 Sugiyama et al.47 N/A pHBV pol dead This paper N/A pSPT18 Roche Cat#11175025910 pSPT18-HBVRNA This paper N/A pSPT18-hGAPDH This paper N/A pCMV-globin Norm Kurosaki et al.49; Zhang et al.50 N/A pCMV-globin Ter39 Kurosaki et al.49; Zhang et al.50 N/A pCMV-mMUP This paper N/A pLKO-shUPF1 This paper N/A pLKO-shUPF2 This paper N/A pLKO puro Addgene Cat#8453 Software and algorithms GraphPad Prism9 GraphPad N/A e2 iScience 28, 112460, May 16, 2025

Article Title: Cell-intrinsic regulation of HBV RNAs by the non-sense mediated mRNA decay pathway controls viral replication
Article Snippet: All primer sets and dsDNAs are listed in Table S1. iScience 28, 112460, May 16, 2025 e3 In vitro transcription of HBV RNA Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly (A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Mucosal boosting increases protective efficacy of an influenza vaccine in mice
Article Snippet: A standard curve was generated with log dilution from 10 7 copies to 100 copies of in vitro transcribed M gene using the AmpliCap-Max T7 High Yield Message Maker Kit (CellScript).

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: Capped and polyadenylated viral RNAs (pgRNA, pgRNA pol dead, 2.4 knt, 2.1 knt, Small S and X RNA) were produced in vitro from T7 promoter-driven plasmids, which were pcDNA 3.1 constructs containing the respective region, using an AmpliCap-Max T7 High Yield Message Maker Kit (CellScript) or mMESSEGE mMACHINETM T7 transcription kit (Thermo Fisher Scientific) and a Plus Poly(A) Polymerase Tailing Kit (CellScript) according to the manufacturer’s protocol.

Article Title: Zinc gluconate protects against plant virus infection in tomato and <i>Nicotiana </i><i>benthamiana</i> plants
Article Snippet: We performed in vitro transcription using 2 μg samples of this template DNA using the AmpliCap-Max T7 High Yield Message Maker Kit (CELLSCRIPT, Madison, WI, USA).

Article Title: A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.
Article Snippet: SARS-CoV-2 N (nucleocapsid) gene was cloned into a pcDNA3.1 expression plasmid and transcribed using an AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript) to be utilized as a standard. qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: A rich conformational palette underlies human Ca V 2.1-channel availability
Article Snippet: In vitro cRNA transcription was performed with the AmpliCap-Max T7 High Yield Message Maker Kit (Cellscript); RNA was stored at −80 °C in RNA storage solution (Thermo Fisher Scientific). α 1A subunits were coexpressed with rabbit α 2 δ-1 ( CACNA2D1 , Uniprot P13806) and either rat β 2a ( CACNB2A , UniProt Q8VGC3) or rabbit β 3 ( CACNB3 , Uniprot P54286) subunits.

Article Title: Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication
Article Snippet: AmpliCap-Max T7 High Yield Message Maker Kit , CellScript , Cat#C- ACM04037.



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